Wang X, Cheng H, Zhao J, et al, Long noncoding RNA DLGAP1-AS2 promotes tumorigenesis and metastasis by regulating the Trim21/ELOA/LHPP axis in colorectal cancer, Mol Cancer, 2022. (IF: 27.7)

Abstract: This article explores the role of long noncoding RNA DLGAP1-AS2 in colorectal cancer (CRC), using quantitative RT-PCR to detect the expression of DLGAP1-AS2, and conducting in vitro and in vivo experiments to assess its impact on the growth and metastasis of CRC. The mechanism of action was revealed through RNA immunoprecipitation, RNA sequencing, luciferase reporter gene analysis, and chromatin immunoprecipitation. The study found that DLGAP1-AS2 interacts with Elongin A (ELOA), promoting the ubiquitination and degradation of ELOA, thereby promoting the occurrence and metastasis of CRC. The research also discovered that DLGAP1-AS2 inhibits the transcriptional activation of the LHPP gene mediated by ELOA, reduces the expression of LHPP, and blocks the inhibition of the AKT signaling pathway. Furthermore, it was demonstrated that DLGAP1-AS2 is bound and stabilized by the cleavage and polyadenylation specificity factor (CPSF2) and the cleavage stimulation factor (CSTF3).

摘要：本文探讨了长非编码RNA DLGAP1-AS2在结直肠癌（CRC）中的作用，使用定量RT-PCR检测DLGAP1-AS2的表达，并进行体外和体内实验来评估其对CRC生长和转移的影响。通过RNA免疫沉淀、RNA测序、荧光素酶报告基因分析、染色质免疫沉淀等揭示其作用机制。研究发现DLGAP1-AS2与Elongin A (ELOA)相互作用，通过促进ELOA的泛素化和降解来促进CRC的肿瘤发生和转移。研究发现DLGAP1-AS2抑制ELOA介导的LHPP基因的转录激活，降低LHPP表达，阻断AKT信号通路的抑制。此外，还证明DLGAP1-AS2被剪接和多聚腺苷酸化特异性因子（CPSF2）和剪接刺激因子（CSTF3）结合并稳定。