Hu QX, Ottestad-Hansen S, Holmseth S, et al, Expression of Glutamate Transporters in Mouse Liver, Kidney, and Intestine. J Histochem Cytochem, 2018. (IF:3.9)

Abstract: This study investigated the distribution of excitatory amino acid transporter (EAAT) subtypes 1-3 in the mouse liver, kidney, and intestine. The study discovered that EAAT3 protein levels are high in the brush borders of the distal small intestine and renal proximal tubules. No significant levels of EAAT1 or EAAT2 were detected in the mouse kidney or intestine. In the liver, only EAAT2 and its two C-terminal splice variants were expressed, located in the plasma membranes of hepatocytes around the central veins. These hepatocytes also express glutamine synthetase. Conditional deletion of EAAT2 in the liver did not result in noticeable neurological issues or fatty liver development.

摘要：这项研究探讨了小鼠肝脏、肾脏和肠道中兴奋性氨基酸转运蛋白（EAAT）1-3亚型以及谷氨酸-半胱氨酸交换器（xCT）的分布情况。研究结果表明，在小鼠的远端小肠和肾脏近曲小管的刷状缘中，EAAT3蛋白的水平较高。而在小鼠的肾脏和肠道中，EAAT1和EAAT2的水平并未显著检测到。在肝脏中，只检测到EAAT2及其两种C末端剪接变体的表达，并且这些EAAT2位于肝静脉周围肝细胞的质膜中。这些肝细胞也表达谷氨酰胺合成酶。值得注意的是，肝脏中EAAT2的条件性删除并没有导致明显的神经系统问题或脂肪肝的发展。这些发现有助于我们更好地理解谷氨酸转运蛋白在不同器官中的作用及其在疾病中的潜在影响。